Precision Extruding Deposition for Freeform Fabrication of PCL and PCL-HA Tissue Scaffolds Overall, the present review affords an insight into the properties and applications of PCL in various tissue engineering applications. Further, various forms of PCL scaffolds such as porous, films and fibrous have been discussed along with the stem cells and their sources employed in various tissue repair strategies. In this narrative review on polycaprolactone ( PCL), we have discussed in detail about the synthesis of PCL, various properties and most recent advances of using PCL and PCL blended with either natural or synthetic polymers and ceramic materials for TE applications. Recent advances in TE have led to the development of suitable scaffold architecture for various tissue defects. Siddiqui, Nadeem Asawa, Simran Birru, Bhaskar Baadhe, Ramaraju Rao, Sreenivasaīiomaterial-based scaffolds are important cues in tissue engineering (TE) applications. PCL-Based Composite Scaffold Matrices for Tissue Engineering Applications. This biocompatible fluorescent composite fibrous scaffold can support in vitro cell growth and also has the potential to act as an optical probe for tissue engineering application in vitro and in vivo. Toxicity study indicated FNDs/ PCL scaffolds were nontoxic. The obtained scaffolds not only exhibited photoluminescence, but also showed reinforced mechanical strength. The FNDs were then mixed with PCL polymer and subsequently electrospun into FNDs/ PCL fibrous scaffolds. In this letter, fluorescent nanodiamonds (FNDs) were prepared after surface passivation with octadecylamine. Biodegradable PCL fibrous scaffold can work as an appropriate substrate for tissue regeneration. Polycaprolactone ( PCL) has been widely studied for biological applications. Therefore, PCL/OSP scaffolds had a high potential to be employed in the bone tissue engineering.įluorescent composite scaffolds made of nanodiamonds/ polycaprolactoneĬao, Li Hou, Yanwen Lafdi, Khalid Urmey, Kirk There was no significant cytotoxicity of the prepared scaffolds towards MG-63 cells, and all the scaffolds showed a well ALP activity. OSP particles are dispersed in the polymer matrix, which helped to improve the degree of crystallinity and mineralization ability of the scaffolds. All the 3D printed scaffolds hold a highly porosity and interconnected pore structures. The PCL and PCL/OSP scaffolds were prepared using additive manufacturing process.
In this work, oyster shell powder (OSP) was used as the bio-filler and combined with polycaprolactone ( PCL) through melt blending methodology. Luo, Wenfeng Zhang, Shuangying Lan, Yuewei Huang, Chen Wang, Chao Lai, Xuexu Chen, Hanwei Ao, Ningjian All rights reserved.ģD printed porous polycaprolactone/oyster shell powder ( PCL/OSP) scaffolds for bone tissue engineering Additionally, due to the alginate component in the fibrous scaffold, they showed significantly enhanced hydrophilic behaviour, water absorption (âˆ❈-fold) and significant biological activities (âˆ❁.6-fold for cell viability at 7 days, âˆ❂.3-fold for ALP activity at 14 days and âˆ❆.4-fold for calcium mineralisation at 14 days) compared with those of a pure PCL fibrous scaffold. With the reinforcing micro-sized PCL struts, the elastic modulus of the PCL/alginate scaffold was significantly improved versus a pure PCL scaffold. To show the feasibility of the scaffolds for hard-tissue regeneration, the scaffolds were assessed not only for physical properties, including hydrophilicity, water absorption, and tensile and compressive strength, but also in vitro cellular responses (cell viability and proliferation) and osteogenic differentiation (alkaline phosphatase (ALP) activity, and mineralisation) by culturing with pre-osteoblasts (MC3T3-E1 cells). Using the process, we obtained polycaprolactone ( PCL)/alginate composite scaffolds, consisting of electrospun PCL/alginate fibres and micro-sized PCL struts, with mean pore sizes of 821 ± 55 μm. To overcome these shortcomings, we suggest a new composite system, which uses a combination method of wet electrospinning, rapid prototyping and a physical punching process.
However, poor mechanical properties, low controllability of various shapes and difficulties in obtaining controllable pore structure have been obstacles to their use in hard-tissue regeneration. Micro/nanofibrous scaffolds have been used widely in biomedical applications because the micro/nano-scale fibres resemble natural extracellular matrix and the high surface-to-volume ratio encourages cellular activities (attachment and proliferation). Three-dimensional electrospun polycaprolactone ( PCL)/alginate hybrid composite scaffolds.
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